Composite
Part:BBa_K607034
Designed by: Olesja Popow Group: iGEM11_Groningen (2011-09-18)
PlasB_LasR_antipBad/araC with 1.0RBS
This part is a member of LasR autoinducing loop family.
LasR protein without degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 1.0 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 195
Illegal NheI site found at 1052 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1113
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 642
Illegal AgeI site found at 839
Illegal AgeI site found at 1278 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1295
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Categories
Parameters
None |